Protein DENEDDYLASE1 counters automodification of neddylating enzymes in Arabidopsis

In eukaryotes, the conjugation of the ubiquitinlike protein NEDD8 onto protein targets is an important post-translational modification. Little is known about the role of other deneddylating enzymes the role of neddylation and deneddylation of their substrates.

Phosphoproteome Profiling Reveals Molecular Mechanisms of Growth-Factor-Mediated Kinase Inhibitor Resistance in EGFR-Overexpressing Cancer Cells

Here we show that epidermal growth factor receptor (EGFR) inhibition by Gefitinib is counteracted by growth factors, notably FGF2, and we assessed the global molecular consequences of this resistance at the proteome and phosphoproteome level in A431 cells.

Chemical proteomics and structural biology define EPHA2 inhibition by clinical kinase drugs

Here, we used chemical proteomics to survey 235 clinical kinase inhibitors for their kinase selectivity and identified 24 drugs with submicromolar affinities for EPHA2.

Ethylene glycol improves electrospray ionization efficiency in bottom-up proteomics

Here, we systematically evaluated the concept by screening about 30 compounds with various physico-chemical properties as LC additives.

MALDI‐TOF and nESI Orbitrap MS/MS identify orthogonal parts of the phosphoproteome

In this study, we compared tandem mass spectrometry (MS/MS) on MALDI-TOF nESI Orbitrap instruments with respect to their ability to identify phosphopeptides from complex proteome digests.

Chemical Proteomics Reveals Ferrochelatase as a Common Off-target of Kinase Inhibitors

The enzyme ferrochelatase (FECH) catalyzes the conversion of protoporphyrin IX into heme and was recently found to be an off-target of the BRAF inhibitor Vemurafenib, likely explaining the phototoxicity associated with this drug in melanoma patients. This raises the question of whether FECH binding…

Ortner-Preis für WZW-Absolventin

Bei der Akademischen Jahresfeier der Technischen Universität München (TUM) wurde bekannt gegeben, welche TUM-Nachwuchsforscher 2015 zu den Besten zählen. Mit dabei ist Master-Absolventin Jana Zecha vom Lehrstuhl für Proteomik und Bioanalytik am Wissenschaftszentrum Weihenstephan für Ernährung,…